Xie Group FOR Genetic Circuit Engineering

Tsinghua National Lab - Bioinformatics Division - Center for Synthetic and Systems Biology

Congratulations to Dacheng & Shuguang on the split dCas9 paper!

We constructed logic AND circuits by integrating multiple split dCas9 domains, which is useful to reduce the size of synthetic circuits. In addition, we engineered sensory switches by exchanging split dCas9 domains, allowing differential regulations on one gene, or activating two different genes in response to cell-type specific microRNAs. (Ma D. et al., Nature Communications, 2016) Read More...

Congratulations to Tingting on the microRNA cluster paper!

We have developed a hierarchical cloning method for efficient construction of synthetic miRNA cluster containing up to 18 miRNA precursors, which provides a modular and powerful tool for multiplex gene knockdown at posttranscriptional level for complex functional genomic studies. (Wang T. et al., ACS Synthetic Biology, 2016) Read More...

Congratulations to Bing and Ye on the microRNA-ceRNA paper!

A minimum ceRNA model to quantitatively analyze the behavior of the ceRNA regulation and the corresponding synthetic gene circuit was implemented in cultured human cells to validate model predictions. (Yuan Y. et al., PNAS, 2015) Read More...

Congratulations to Yinqing, Yun, He and Weixi on the TALER paper!

A transcription repressor toolkit made of TALE DNA binding scaffold for genetic circuit engineering, which enables applications in mammalian cells such as identification of cancerous cells. (Li. Y., et al., Nature Chemical Biology, 2015) Read More...

Transcripts for combined synthetic microRNA and gene delivery

MicroRNAs (miRNAs) are a class of short endogenous non-coding RNAs that post-transcriptionally regulate messenger RNAs (mRNAs). In nature, miRNAs could be produced from their independent transcription units in either intergenic regions or intragenic regions. Intragenic miRNAs and their host mRNAs are produced from the same transcript by microprocessor and spliceosome complex respectively. However, the timing and relationship of microprocessing and splicing are not well understood. In this paper, we engineer and study in mammalian cells a range of synthetic intragenic miRNAs co-expressed with their host genes. These results shed additional light on RNA processing event and provide insight into engineering transcripts customized for combined delivery and use in sophisticated circuits.

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