Xie Group FOR Genetic Circuit Engineering

Tsinghua National Lab - Bioinformatics Division - Center for Synthetic and Systems Biology

Transcripts for combined synthetic microRNA and gene delivery

MicroRNAs (miRNAs) are a class of short endogenous non-coding RNAs that post-transcriptionally regulate messenger RNAs (mRNAs). In nature, miRNAs could be produced from their independent transcription units in either intergenic regions or intragenic regions. Intragenic miRNAs and their host mRNAs are produced from the same transcript by microprocessor and spliceosome complex respectively. However, the timing and relationship of microprocessing and splicing are not well understood. In this paper, we engineer and study in mammalian cells a range of synthetic intragenic miRNAs co-expressed with their host genes. These results shed additional light on RNA processing event and provide insight into engineering transcripts customized for combined delivery and use in sophisticated circuits.